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	Provedor de dados BABT (6) BJM (1) Autor Alegre,Ranulfo Monte (7) Santos,Lucielen Oliveira dos (2) Anschau,Andréia (1) Bastos,Reinaldo Gaspar (1) Bezerra,José Raniere Mazile Vidal (1) Braga,Ana Luíza de Mattos (1) Calheiros,Chrissana Santos (1) Coelho,Narjara (1) Flôres,Simone Hickmann (1) Gonzales,Tatiane Araujo (1) Justo,Oselys Rodriguez (1) Lima,Álvaro Silva (1) Padilha,Giovana da Silva (1) Perez,Victor Haber (1) Rigo,Maurício (1) Santana,José Carlos Curvelo (1) Tambourgi,Elias Basile (1) Úbeda,Beatriz Torsani (1) Mais... Palavra-chave Fermentation (3) Glutathione (2) Alkaloid (1) Amino Acids (1) Anticancer (1) Aqueous two phase system (1) Batch fermentation (1) Biodegradation (1) Biosurfactant (1) Burkholderia cepacia (1) Candida parapsilopsis (1) Catechol (1) Characterization (1) Diesel oil (1) Emulsifier stability (1) Equation of Haldane (1) Fed Batch Culture (1) Growth kinetics (1) Lactococcus lactis (1) Lantibiotic (1) Mais... Tipo do documento Info:eu-repo/semantics/article (7) Ano 2013 (1) 2012 (2) 2010 (1) 2009 (1) 2007 (1) 2003 (1) Mais... País Brazil (7) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol BABT Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte. This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017 A modified method for the turbidimetric assay of nisin BABT Flôres,Simone Hickmann; Braga,Ana Luíza de Mattos; Alegre,Ranulfo Monte. Nisin is an antibacterial peptide produced by Lactococcus lactis subsp. lactis that shows a broad spectrum of inhibitory activity against gram-positive microorganism including bacterial spores. Methods for nisin activity estimation in solution is redefined. In this work a new turbidimetric assay method has been described making possible to assay nisin satisfactorily in total incubation period of approximately six hours. Tipo: Info:eu-repo/semantics/article Palavras-chave: Lantibiotic; Nisin; Lactococcus lactis; Turbidimetric assay. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300020 Catechol biodegradation kinetics using Candida parapsilopsis BABT Rigo,Maurício; Alegre,Ranulfo Monte; Bezerra,José Raniere Mazile Vidal; Coelho,Narjara; Bastos,Reinaldo Gaspar. This study evaluated the biodegradation of catechol by a yeast strain of Candida parapsilopsis in standard medium in Erlenmeyer flasks. Results shown that the highest concentration of catechol caused the longer lag period, demonstrating that acclimatized cultures could completely degrade an initial catechol concentration of 910 mg/L within 48 h. Haldane's model validated the experimental data adequately for growth kinetics over the studied catechol concentration ranges of 36 to 910 mg/L. The constants obtained for this model were µmax = 0.246 h-1, Ks = 16.95 mg/L and Ki = 604.85 mg/L. Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida parapsilopsis; Biodegradation; Catechol; Growth kinetics; Equation of Haldane; Substrate inhibition. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200029 Evaluation of emulsifier stability of biosurfactant produced by Saccharomyces lipolytica CCT-0913 BABT Lima,Álvaro Silva; Alegre,Ranulfo Monte. Surface-active compounds of biological origin are widely used for many industries (cosmetic, food, petrochemical). The Saccharomyces lipolytica CCT-0913 was able to grow and produce a biosurfactant on 5% (v/v) diesel-oil at pH 5.0 and 32ºC. The cell-free broth emulsified and stabilized the oil-in-water emulsion through a first order kinetics. The results showed that the initial pH value and temperature influenced the emulsifier stability (ES), which was the time when oil was separated. The biosurfactant presented different stabilization properties for vegetable and mineral oil in water solution, despite the highest values of the ES occurring with vegetable oil. The biosurfactant presented smallest ES when compared to commercial surfactants; however, this... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Emulsifier stability; Fermentation; Vegetable oil; Diesel oil. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200004 Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization BABT Padilha,Giovana da Silva; Santana,José Carlos Curvelo; Alegre,Ranulfo Monte; Tambourgi,Elias Basile. This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Burkholderia cepacia; Aqueous two phase system; Partitioning; Characterization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002 Glutathione production using magnetic fields generated by magnets BABT Santos,Lucielen Oliveira dos; Gonzales,Tatiane Araujo; Úbeda,Beatriz Torsani; Alegre,Ranulfo Monte. The objective of this work was to study the production of GSH by Saccharomyces cerevisiae ATCC 7754 in a fermentor (5 L) using a cell recycle system with magnets. The fermentation conditions were 20°C, 500 rpm, 5% (v/v) of inoculum, pHinitial 5, 1.1 vvm aeration and total fermentation time of 72 h. The time of application of MF ranged from 24, 48 or 72 h. In comparison to the control experiment, the best results were obtained with 72 h of application of MF. The cell concentration reached 19.5 g/L and GSH concentration was 271.9 mg/L that corresponded to an increase of 2.63 and 32.1% compared to the control experiment, respectively. Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione; Magnetic field; Saccharomyces cerevisiae; Batch fermentation. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600016 Screening of Metarhizium spp. strains for anticancer indolizidine alkaloid production and its rapid detection by MS analysis BJM Justo,Oselys Rodriguez; Calheiros,Chrissana Santos; Perez,Victor Haber; Alegre,Ranulfo Monte. Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were studied regarding their ability to produce an anticancer indolizidine alkaloid. The culture process was carried out in Shaken flask at 26ºC and 200 rpm using three different culture medium containing oat meal extract supplemented with glucose and DL-lysine or Czapek culture medium. The mycelial extracts produced by Metarhizium spp. cultures were directly submitted to electrospray ionization mass spectrometry (ESI-MS) analysis and the highest alkaloid concentration (approximately, 6 mg.L-1) was reached when M. anisopliae 3935 was tested. Tipo: Info:eu-repo/semantics/article Palavras-chave: M. anisopliae; M. flavoviride; Anticancer; Alkaloid; Fermentation. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400035 Registros recuperados: 7 Primeira ... 1 ... Última

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